ChIPseq

Chromatin immunoprecipitation sequencing (ChIPseq) is a method used to analyze DNA-protein interactions. Typical targets are histone protein modifications and transcription factors (TFs).

Pricing

QC + library prep + sequencing
(40M 50bp read pairs)
DFCI/BWH: $300
External: $360
Peak calling + QC analysis (CHIPS)
+$20/sample

Turnaround

2-3 weeks

Get started

place a request through iLab >

DNA QC

DNA is assayed by qubit and/or bioanalyzer to determine concentration and appropriate size distribution for Illumina sequencing (200-600bp).

Analytical Goals

Genome-wide analysis of histone modifications or TF binding.

Sample Requirements

DNA Amount: >1ng is ideal –we can work with less
DNA Size distribution: 200-600bp
Minimum Input: below detection limit of qubit and bioA
MBCF Optimal Input Amount: 2ng
Volume: >10ul